Journal: Experimental and Therapeutic Medicine

Article Title: Involvement of neurotrophic signaling in doxorubicin-induced cardiotoxicity

doi: 10.3892/etm.2019.8276

Figure Lengend Snippet: Effects of DOX on the expression of BDNF and NGF in the serum and heart. (A) BDNF concentration in the serum. (B) BDNF mRNA levels in the heart. (C) BDNF protein expression levels in the heart. (D) NGF concentration in the serum. (E) NGF mRNA levels in the heart. (F) NGF protein levels in the heart. Data are expressed as the mean ± standard deviation (n=8–9). *P<0.05 and **P<0.01 compared with Con. NGF, nerve growth factor; BDNF, brain derived neurotrophic factor; DOX, doxorubicin; Con, control.

Article Snippet: Determination of serum BDNF and NGF levels ELISA kits were purchased from Wuhan USCN Business Co., Ltd. and were used to determine plasma levels of BDNF (cat. no. SEA011Ra) and NGF (cat.no.

Techniques: Expressing, Concentration Assay, Standard Deviation, Derivative Assay

Journal: Communications Biology

Article Title: Norepinephrine regulates epithelial-derived neurotrophins expression and sensory nerve regeneration through ADRB2 receptor

doi: 10.1038/s42003-025-07903-5

Figure Lengend Snippet: A The NGF mRNA expression of HCECs with different NE concentration treatments. B The expression of various neurotrophins in HCECs with 10 μM NE treatment was detected with qPCR. The NGF and GDNF expression of HCECs treated with 10 μM NE were detected with qPCR ( C ), ELISA ( D ) and immunofluorescence staining ( E ). F The fluorescence intensity of NGF and GDNF in HCECs treated with 10 μM NE was quantified by Image J software. Corneal NGF and GDNF expressions were measured with qPCR ( G ) and ELISA ( H ). I Immunofluorescence staining of NGF and GDNF in the cornea. J The fluorescence intensity of NGF and GDNF was quantified by Image J software. Data are representative of the mean ± SD. ** p < 0.01, *** p < 0.001.

Article Snippet: Following this, the NGF and GDNF were detected by using ELISA kits specific for mouse NGF (USCN Life Science Inc., Wuhan, China) and mouse GDNF (sourced from Abcam) to measure the levels of NGF and GDNF in each aliquot.

Techniques: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Fluorescence, Software

Journal: Communications Biology

Article Title: Norepinephrine regulates epithelial-derived neurotrophins expression and sensory nerve regeneration through ADRB2 receptor

doi: 10.1038/s42003-025-07903-5

Figure Lengend Snippet: DM mice were intraperitoneally injected with 6-OHDA and subsequently debrided the central corneal epithelium. The corneas of healthy, DM, and 6-OHDA-treated DM mice were collected at 48 h after epithelial debridement. A NGF and GDNF mRNA transcripts were monitored with qPCR ( n = 3 mice per group). B NGF and GDNF protein levels were measured with an ELISA (8 corneas pooled per group). C Immunofluorescence staining of NGF and GDNF in the cornea. D The fluorescence intensity of NGF and GDNF in the corneal epithelium was quantified by Image J software. E Mouse corneal nerves were stained with the Alexa Fluor® 488-labeled α-βIII-tubulin antibody at 48 h post wounding. F The subbasal nerve fibers were counted with Image J software ( n = 4 corneas per group). G Corneal sensations were measured with a Cochet-Bonnet esthesiometer ( n = 4 mice per group). Data are representative of the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Following this, the NGF and GDNF were detected by using ELISA kits specific for mouse NGF (USCN Life Science Inc., Wuhan, China) and mouse GDNF (sourced from Abcam) to measure the levels of NGF and GDNF in each aliquot.

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Fluorescence, Software, Labeling

Journal: Communications Biology

Article Title: Norepinephrine regulates epithelial-derived neurotrophins expression and sensory nerve regeneration through ADRB2 receptor

doi: 10.1038/s42003-025-07903-5

Figure Lengend Snippet: A Relative expression of ADRs in the mouse cornea. B – E NGF and GDNF mRNA transcripts and protein levels were detected with qPCR and ELISA in HCECs treated with PBS, NE, NE and atenolol (ATE), and NE and ICI 118, 551 (ICI). F Scheme of indirect cell culture of TKE2 cells and TG neurons. G Representative TG neurite outgrowth incubated with the various conditioned media of TKE2 cells treated with PBS, NE, NE and ATE, and NE and ICI. The cultured TG neurons were stained with Alexa Fluor® 488-labeled βIII-tubulin antibody. H The TG neurite outgrowth was analyzed with Neuro J software. Data are representative of the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Following this, the NGF and GDNF were detected by using ELISA kits specific for mouse NGF (USCN Life Science Inc., Wuhan, China) and mouse GDNF (sourced from Abcam) to measure the levels of NGF and GDNF in each aliquot.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Incubation, Staining, Labeling, Software

Journal: Communications Biology

Article Title: Norepinephrine regulates epithelial-derived neurotrophins expression and sensory nerve regeneration through ADRB2 receptor

doi: 10.1038/s42003-025-07903-5

Figure Lengend Snippet: DM mice were performed with corneal epithelial debridement and topically applied with the eye drop containing 10 μM ICI 118, 551 (ICI). A , B Mouse corneal epithelial defects were monitored with fluorescein staining and calculated with Image J software ( n = 5 mice per group). C , D Mouse corneal nerves were stained and the subbasal nerve fibers were counted with Image J software at 48 h post wounding ( n = 4 corneas per group). E Corneal sensations were measured with a Cochet-Bonnet esthesiometer at 48 h post wounding ( n = 5 mice per group). Corneal NGF and GDNF expressions were detected with qPCR ( n = 3 mice per group) at 48 h post wounding ( F ) and immunofluorescence staining ( G ). H The fluorescence intensity of NGF and GDNF in the corneal epithelium was quantified by Image J software. Data are representative of the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Following this, the NGF and GDNF were detected by using ELISA kits specific for mouse NGF (USCN Life Science Inc., Wuhan, China) and mouse GDNF (sourced from Abcam) to measure the levels of NGF and GDNF in each aliquot.

Techniques: Staining, Software, Immunofluorescence, Fluorescence

Journal: Neural Regeneration Research

Article Title: Enteric glia mediate neuronal outgrowth through release of neurotrophic factors

doi: 10.3969/j.issn.1673-5374.2012.028.001

Figure Lengend Snippet: Enzyme-linked immunosorbent assay (ELISA) analysis of neurotrophic factor release of enteric glia (EG) cells. EG cells were maintained in a standard culture medium containing 10% fetal bovine serum for 24 hours. Medium was changed to one with 2% fetal bovine serum, cells were cultured continually for another 48 hours, and then medium was removed and examined for neurotrophic factor concentration by ELISA. EG cells released NGF, BDNF and GDNF under controlled conditions, with their respective concentrations reaching 250, 96.4 and 53.8 pg/mL respectively. All bars represent mean ± SEM of five independent experiments. NGF: Nerve growth factor; BDNF: brain-derived neurotrophic factor; GDNF: glial cell line-derived neurotrophic factor.

Article Snippet: Subsequently, the cells were centrifuged and the growth factors were measured in the supernatant using commercially available ELISA kits for NGF, BDNF, NT-3 and GDNF (Promega, Madison, WI, USA) using the protocols described by the manufacturer.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Concentration Assay, Derivative Assay